During illness, the stiffness or viscosity of cells can change. Tom Evers demonstrated this by measuring such properties of human immune cells for the first time. 'The stiffness of certain cells could be a way to make a diagnosis,' Evers said. He defended his thesis on March 26th.
Within a tumor, cells grow close together in tissue where they do not belong. Therefore, there's more pressure from packed cells. Cells react to this by reinforcing their structure, just as our bones become stronger when subjected to more stress. The stiffness of cells likely also plays a role in leaky blood vessels. Blood vessel cells may become less rigid or even somewhat fluid. Viruses like Ebola are deadly due to hemorrhagic fever, where blood vessels begin to leak. Tom Evers succeeded in measuring the stiffness of cells for the first time.
To Siberia
As a student of Biomedical Sciences, Evers found biophysics so interesting that he was willing to go to Siberia for it. 'It was sometimes minus 40 degrees in Tomsk, but I also experienced plus 40.' He had a great time there in 2016/17, when he completed the first half of his double master's degree. The other half was biomedical in nature, back in his hometown of Maastricht. 'In Leiden, I could combine both fields.'
A macrophage must be able to deform
Evers could express his adventurous side well in Alireza Mashaghi's lab at LACDR. 'We study the mechanical properties of cells: their stiffness and viscosity. These are extremely important in, among other things, the immune response to disease.' For example, a macrophage that engulfs a pathogen must be able to deform around it smoothly.
These mechanical properties also play a role in cancer. 'In a tumour, some cells become softer, less rigid. These cells can more easily migrate through the body, which happens in metastatic cancer.' In leukemia, where monocytic immune cells are affected, their mechanical properties change. 'If we can measure that accurately, the stiffness of such cells could be a way to diagnose the disease.' Cell stiffness then becomes a so-called biomarker.
With an optical tweezer, he stretched a cell
Evers worked with optical tweezers, among other tools. With this device, scientists can, for example, hold a strand of DNA, pull on it, and then measure how tightly it is wound. 'I modified the tweezers so that you can sandwich a cell between two glass beads. By pushing the beads together with a certain force and observing the stretch the cell undergoes, we determine the stiffness of the cell.'
Near tumours, something strange happens
Near tumours, something strange happens with macrophages. While some of them clear tumour cells as hoped, others surround the tumour and actually protect the tumour cells. Evers wanted to prove whether these different subtypes of immune cells are identifiable by their stiffness. He extracted macrophages from healthy mouse breast tissue and tumor-associated macrophages from mice with breast cancer. 'The macrophages protecting the tumour had higher rigidity.'
A new field in biology
'With our mechanobiology, we aim to introduce a new field within biology,' says Tom Evers about his work. When studying genes, biologists talk about genomics. If it's about proteins, they call it proteomics. Research into metabolism is metabolomics. Evers' results show that there are even more relevant aspects. 'Mechanomics adds the dimension of mechanical properties such as stiffness and viscosity.'
The adventure isn't over
Evers' supervisor Alireza Mashaghi is very pleased with the work of his PhD candidate. 'He introduced mechanics into the research field of immunology, thus contributing to the emergence of the field of mechanoimmunology. He managed to apply techniques to measure the mechanical properties of immune cells during illness.'
End of this adventure, is Evers moving on to the next? No, the adventure isn't over yet. 'I'm staying with the department, now as a postdoc researcher. I've spent a long time developing the technology, and the results only came at the end. I'm now researching diseases where blood vessels leak, which must also have to do with the mechanical properties of blood vessels.' Viruses like Ebola are deadly due to hemorrhagic fever with leaking blood vessels. This is also related to changing mechanical properties of blood vessel cells. So, there's a lot for single-cell mechanobiologist Evers to discover.
Tom Evers received his PhD on 26 March for the thesis Single-cell mechanics for disease biology and pharmacology.
Text: Rianne Lindhout
Image above article: Flickr/Ed Uthman
