Nunawading Highway Patrol officers are investigating a collision in Mulgrave yesterday morning (13 February), Victoria Police say.
It is believed a motorcycle clipped a grey Toyota RAV4 and crashed into the back of a stationary truck on the Monash Freeway near Springvale Road about 6.10am.
The male rider, a 35-year-old Langwarrin man, was conveyed to hospital with serious but non-life threatening injuries.
The truck driver, a 48-year-old Cranbourne North man and the driver of the car, a 21-year-old man from Rowville, were both uninjured and stopped at the scene to assist.
The exact circumstances surrounding the collision are yet to be determined.
Police are investigating whether lane splitting may have been a contributing factor.
Investigators are appealing for anyone who may have dashcam footage of the incident or witnessed any erratic driving prior to the collision to come forward.
The motorcycle involved is a black and yellow 2013 Suzuki VZR-180.
Anyone with any information is urged to contact Crime Stoppers.