Police from Endeavour Hills and Narre Warren have charged two men as part of an investigation into the death of a kangaroo in Lysterfield South last month.
The pair were charged on Thursday following the execution of warrants at two residential homes, in Ferntree Gully Road, Scoresby and Bourke Street in Ringwood.
The charges follow a report to police that a kangaroo was deliberately hit by a car and killed on 23 April.
Officers have been told a Nissan Patrol was seen travelling along Hallam North Road when it is believed the driver deliberately drove toward a group of kangaroos about 10.20pm.
One of the kangaroos died at the scene.
The men, a 22-year-old from Scoresby and a 20-year-old from Ringwood, have been charged with aggravated cruelty to animals and destroying protected wildlife.
They will appear before Dandenong Magistrates' Court on 19 August.
Police also seized a Nissan Patrol from one of the properties.
Authorised Officer's from the Conservation Regulator assisted police during the execution of the warrants.
