Four people have been taken to hospital following a serious crash near Acacia Hills overnight.
Around 12:55am this morning, the Joint Emergency Services Communication Centre received a report of a crash between a road train and a Toyota Prado on the Stuart Highway.
The crash was reported to have occurred about 2km north of a service station in Acacia Hills.
The vehicle is believed to have been travelling south when it collided with the rear trailer of a road train turning north out of Acacia Quarry.
Four adults, two men and two women were found injured at the scene.
All four were conveyed to Royal Darwin Hospital via St John Ambulance with one female passenger in a critical condition.
The road train initially stopped before continuing to a truck yard in Humpty Doo.
Police located the road train at a business in Humpty Doo where the driver returned a positive drug test and was arrested.
Emergency service workers are still at the scene of the crash. There are diversions around the crash scene. The Stuart Hwy is expected to be open by 11:00am.
The Major Crash Unit is investigating and anyone with information or dash cam footage is urged to contact police on 131 444 or Crime Stoppers on 1800 333 000 and reference P26132382.