Southern Metro Region Divisional Response Unit detectives arrested three people after a large cannabis crop house was located in Tooradin yesterday.
Police executed a search warrant at a rural property on Hardys Road and seized more than 1200 cannabis plants.
The commercial quantity of the plants has an estimated potential street value of approximately $3.6 million.
It is alleged the property was illegally hooked up to the electricity supply in the street, which required specialist resources to isolate prior to police entering.
A 39-year-old St Albans man and a 43-year-old man of no fixed address allegedly attempted to flee the property on foot but were arrested with assistance of the local Drone Unit the Dog Squad.
They were both charged with cultivate large commercial quantity if cannabis and remanded to appear at Melbourne Magistrates' Court on 17 July.
A 48-year-old Doncaster man was arrested, interviewed and is expected to be charged on summons.