Brimbank Highway Patrol officers are investigating the circumstances surrounding a collision in Albion earlier this month, which has now turned fatal.
Emergency services were called to reports a car and motorcycle collided at the intersection of Ballarat Road and Adelaide Street about 11.20pm on Friday, 5 December.
The rider, a 29-year-old Doncaster man, was taken to hospital with life-threatening injuries where he later died.
The driver of the car, a 22-year-old man from Hillside, was not injured and stopped at the scene.
Investigations are ongoing into the circumstances of the collision.
Anyone who witnessed the incident, with footage or information is urged to contact Crime Stoppers on 1800 333 000 or anonymously at www.crimestoppersvic.com.au