With Artemis II, NASA is taking the science of living and working in space beyond low Earth orbit. While the test flight will help confirm the systems and hardware needed for human deep space exploration, the crew also will be serving as both scientists and volunteer research subjects, completing a suite of experiments that will allow NASA to better understand how human health may change in deep space environments. Results will help the agency build future interventions, protocols, and preventative measures to best protect astronauts on future missions to the lunar surface and to Mars.
Science on Artemis II will include seven main research areas:
ARCHeR: Artemis Research for Crew Health and Readiness
NASA's Artemis II mission provides an opportunity to explore how deep space travel affects sleep, stress, cognition, and teamwork — key factors in astronaut health and performance. While these effects are well-documented in low Earth orbit, they've never been fully studied during lunar missions.
Artemis II astronauts will wear wristband devices that continuously monitor movement and sleep patterns throughout the mission. The data will be used for real-time health monitoring and safety assessments, while pre- and post-flight evaluations will provide deeper insights into cognition, behavior, sleep quality, and teamwork in the unique environment of deep space and the Orion spacecraft.
The findings from the test flight will inform future mission planning and crew support systems, helping NASA optimize human performance for the next era of exploration on the Moon and Mars.
Immune Biomarkers
Saliva provides a unique window into how the human immune system functions in a deep space environment. Tracing changes in astronauts' saliva from before, during, and after the mission will enable researchers to investigate how the human body responds to deep space in unprecedented ways.
Dry saliva will be collected before, during, and after the mission. It will be blotted onto specialized paper in pocket-sized booklets since equipment needed to preserve wet spit samples in space - including refrigeration - will not be available due to volume constraints. To augment that information, liquid saliva and blood samples will be collected before and after the mission.
With these wet and dry saliva samples, scientists will gain insights into how the astronauts' immune systems are affected by the increased stresses of radiation, isolation, and distance from Earth during their deep space flight. They also will examine whether otherwise dormant viruses are reactivated in space, as has been seen previously on the International Space Station with viruses that can cause chickenpox and shingles.
The information gathered from this study, when combined with data from other missions, will help researchers develop ways to keep crew members safe and healthy as we explore farther and travel for longer periods on deep space missions.
AVATAR: A Virtual Astronaut Tissue Analog Response
AVATAR is another important component of NASA's strategy to gain a holistic understanding of how the deep space environment affects humans. Scientists plan to use organ-on-a-chip technology during Artemis II, marking the first time these devices will be used beyond the Van Allen belts.
Roughly the size of a USB thumb drive, the chips will measure how individual astronauts respond to deep space stressors, including extreme radiation and microgravity. The organ chips will contain cells developed from preflight blood donations provided by crew members to create miniature stand-ins, or "avatars," of their bone marrow. Bone marrow plays a vital role in the immune system and is particularly sensitive to radiation, which is why scientists selected it for this study.
A key goal for this research is to validate whether organ chips can serve as accurate tools for measuring and predicting human responses to stressors. To evaluate this, scientists will compare AVATAR data with space station findings, as well as with samples taken from the crew before and after flight.
AVATAR could inform measures to ensure crew health on future deep space missions, including personalizing medical kits to each astronaut. For citizens on Earth, it could lead to advancements in individualized treatments for diseases such as cancer.
AVATAR is a demonstration of the power of public-private partnerships. It's a collaboration between government agencies and commercial space companies: NASA, National Center for Advancing Translational Sciences within the National Institutes of Health, Biomedical Advanced Research and Development Authority, Space Tango, and Emulate.
Artemis II Standard Measures
The crew also will become the first astronauts in deep space to participate in the Spaceflight Standard Measures study, an investigation that's been collecting data from participating crew members aboard the space station and elsewhere since 2018. The study aims to collect a comprehensive snapshot of astronauts' bodies and minds by gathering a consistent set of core measurements of physiological response.
The crew will provide biological samples including blood, urine, and saliva for evaluating nutritional status, cardiovascular health, and immunological function starting about six months before their launch. The crew also will participate in tests and surveys evaluating balance, vestibular function, muscle performance, changes in their microbiome, as well as ocular and brain health. While in space, data gathering will include an assessment of motion sickness symptoms. After landing, there will be additional tests of head, eye, and body movements, among other functional performance tasks. Data collection will continue for a month after their return.
All this information will be available for scientists interested in studying the effects of spaceflight via request to NASA's Life Sciences Data Archive. The results from this work could lead to future interventions, technologies, and studies that help predict the adaptability of crews on a Mars mission.
Radiation Sensors Inside Orion
