Croydon Divisional Response Unit detectives have charged two teenagers following a stabbing in Mooroolbark on 10 March.
It is alleged a group of teens were throwing rocks at the roof of a house on Lincoln Road in Croydon on 9 March about 10.15pm.
The group fled before returning hours later at 2.30am and continued to throw rocks at the victim's property.
The victim got into his vehicle and chased the group to Manchester Road in Mooroolbark where an altercation occurred.
It's alleged a member of the group used a machete to slash the victim before fleeing.
The 64-year-old man attended hospital with serious injuries and police were called.
Investigators executed four search warrants in Mooroolbark on 17 March, arresting three teenagers.
Two boys, 17-year-old and 15-year-old from the Yarra Ranges area, have been charged with intentionally causing serious injury, recklessly causing serious injury, common law assault, affray and possessing a prohibited weapon.
Both will appear at a children's court at a later date.
A 16-year-old boy from the Yarra Ranges area was released pending further enquiries.