Monash researchers develop a test that determines who is immune and who will develop a serious

Monash University

Monash scientists are repurposing technology they recently developed to test for a patient’s immunity to allergens and influenza – to make a rapid test to determine who has immunity to coronavirus; remains infectious; andis at risk of developing a severe form of the disease.

The researchers will start receiving cell samples next week from colleagues in Melbourne and three main coronavirus hotspots – Italy, China and New York. There is a growing need to identify immune healthcare workers, minimise absenteeism, and allow workers safely back into the frontline.

The test – being developed by a team led by Associate Professor Menno van Zelm from the Monash University’s Central Clinical School together with Professor Robyn O’Hehir from Central Clinical School and Alfred Health – will also have the capacity to look for differences in the blood of patients with mild disease versus those with a severe infection, in the hope of finding biomarkers that can predict those who may need early medical intervention.

The test, similar to the recently developed test for influenza, looks at what are called ‘memory B lymphocytes’. B lymphocytes are the cells of the immune system that make antibodies to invading pathogens such as viruses.

They form memory cells that remember the same pathogen for faster antibody production in future infections. These are the cells formed after vaccination and that respond quickly when a pathogen is encountered, thereby preventing the disease (e.g. measles, tetanus).

If there is evidence of a large population of memory B cells specific to a pathogen then it is likely that person has been infected some time in the past and will remain immune to the disease.

“It is important that we now move from needing a test that simply tells whether someone is infected – which is the priority now – to needing a test that can determine who is infectious, who is immune, who is going to get a serious case of the disease and who will only develop a mild case of upper airway infection,” Associate Professor van Zelm said.

“This and other tests like it will provide us with a more nuanced approach to managing the disease.”

The Monash team have developed markers for those memory B cells formed after coronavirus infection. By binding these markers to a fluorescent label they can use commonly available equipment called a Fluorescence Activated Cell Sorter to determine the number of memory B cells present in a coronavirus patient, enabling the assessment of their immune status.

They will be also looking at memory B cells taken at various stages, and severity, of coronavirus infection from patients in China, Italy, New York and Australia to look for different markers on these B cells that may become predictors of those who will get a mild version of the disease all the way to those who may need care in ICU.

/Public Release.