About 3.45pm, white and red Freightliner truck towing trailers, was travelling northbound on Bussell Highway, near Harmans Mill Road.
The trailer of the Freightliner truck was involved in a collision with two vehicles travelling southbound - a black Volkswagen Tiguan station sedan, and a gold Subaru Outback station sedan.
Emergency services attended the scene.
The 53-year-old female driver of the gold Subaru was airlifted to Royal Perth Hospital, but later died as a result of critical injuries received.
The 28-year-old female driver of the black Volkswagen hatchback was conveyed to Busselton Hospital for treatment of non-life-threatening injuries.
The 56-year-old male driver of the Freightliner stopped at the scene, and was uninjured.
Major Crash investigators are conducting an examination of the scene and are appealing for any witnesses to call Crime Stoppers on 1800 333 000 or report the information online at www.crimestopperswa.com.au   ;
Dash-cam or mobile phone vision relating to this crash can uploaded directly to investigators via this link: https://wapf.au.evidence.com/axon/community-request/public/mcis2025232&… ;
The WA Police Force continues to urge all motorists to take care on the road. Drive safely and keep in mind the wellbeing of yourself and others.