Anyone who has ever used a microscope knows that it takes time to bring a sample into sharp focus. Each time you move the slide, the image blurs, and you have to stop and carefully turn a knob to bring everything back into clear view. For scientists and clinicians, even if the motion is semi-automated, that time quickly adds up as they work with dozens or hundreds of samples.
Now a team of scientists at Caltech has developed an inexpensive robust fix for this problem that involves little more than a couple of LED lights and some physics-based processing. They describe the new autofocus technique, which they call Digital Defocus Aberration Interference (DAbI), in a paper published online on April 24 in the journal Nature Communications.
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