3D polarization-sensitive OCT imaging of collagen organization within organ systems

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Collagen is essential for tissue integrity and stability, and changes in its intrinsic organization are the key features in several important diseases such as scar formation, heart infarction and cancers. Knowing the organization of collagen patterns is not only important for the early and accurate clinical diagnosis, but also valuable in dermatologic surgical planning for less scarring. However, traditional imaging techniques such as the polarized microscopy and second harmonic generation (SHG) microscopy limit the collagen imaging in the laboratory studies. For example, the polarized microscopy can only provide two dimensional images and requires destructive procedure to the sample that should be avoided as much as possible in the clinic. For the SHG generation imaging technique, the field of view and imaging depth are often too limited to reconstruct macroscopic collagen organizations that reveal the functional architecture of the tissues. Moreover, high incident power of the laser is needed in the SHG imaging to provide sufficient contrast for the collagen, which could damage the targeted living tissues. All these limitations hinder the utilization of the collagen information in the clinical diagnosis.

As a mature medical imaging technology, optical coherence tomography (OCT) provides wide-field, high-resolution, three-dimensional (3D) imaging of living biological tissues without a need of contact or damage to the sample. Therefore, it is an ideal tool of choice for clinical diagnosis and imaged-guided surgery. However, traditional OCT lacks specific contrast for the collagen. Since collagen is highly birefringent, polarization sensitive optical coherence tomography (PSOCT), a functional extension of OCT was therefore developed to image the 3D birefringent material. The challenge is, however, that the traditional PSOCT only provides the accumulated measurement of birefringent information within the sample. That is, using the traditional PSOCT method, the collagen organization embedded deep within the tissue cannot be reconstructed properly.

In a new paper published in Light Science & Application, a team of scientists, led by Professor Ruikang K. Wang from Department of Bioengineering, University of Washington, USA, and co-workers have developed a novel approach that utilizes PSOCT to non-invasively image the depth-resolved collagen organization within the rodent hearts and living human skin. Their method allows scientists or clinicians to “see” the 3D collagen organization within a living tissue without applying any “side-effect” to the sample. More interestingly, this method only requires a single input polarization state to reconstruct the depth-resolved collagen architecture, allowing a cost-effective, flexible and neat PSOCT system design, which is extremely attractive to clinical translation. By providing wide field 3D contrast for the specific tissue non-invasively, this reported method will open up new possibilities for more accurate clinical diagnosis and image-guided surgery, targeting to precision medicine.

This method, called polarization state tracing (PST) method, utilized the trajectory of the output polarization states at the Poincare sphere to extract the local birefringent information at each depth of the sample. By innovatively applying discrete differential geometry (DDG) analysis to the trajectory, local phase retardation and local axis orientation at each depth of the sample can be acquired using a single input polarization state. The scientists summarize the purposes of their development: “We develop the DDG-based PST method to meet the challenges of: (1) a clinical demand for a non-invasive method that can resolve the local birefringent information within a living tissue; and (2) a desire of a method and device being able to meet the requirements for clinical translation.”

Because fibrous collagen shows intrinsic optical anisotropy, local axis orientation mapping can visualize collagen organization by revealing the direction of the collagen alignment. The authors successfully used this method to reconstruct the typical helical-like myocardial fiber structure of a whole mouse heart, observe the disorganization of the myocardial fibers within an infarcted rat heart and visualize the macroscopic architecture of the skin tension lines in a generalized PSOCT system with a single input polarization state. “All these findings are extremely valuable in numerous clinical applications in, for example, early detection of acute myocardial infarction, monitoring of the graft remodeling in the therapeutic treatment of heart infarction, evaluation of scar formation and the imaging-guided plastic surgery, etc. In addition, the significant simplification of the PSOCT imaging system facilitates the clinical translation of this imaging technique, which would provide a new matrix of diagnostic information” they added.

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