Multispecies amplicon sequencing approach for genetic diversity assessments in grassland plant species

Grasslands are widespread and relevant ecosystems at the basis of sustainable roughage production. Plant genetic diversity (PGD; i.e., within-species diversity) is related to many beneficial effects on the ecosystem functioning of grasslands. In their recently published paper, Miguel Loera-Sánchez, Bruno Studer and Roland Kölliker report a set of 12 multispecies primer pairs that can be used for high-throughput PGD assessments in multiple grassland plant species.

The tools presented in this study provide the basis for cost-effective, multispecies genetic diversity assessments in grassland plants and constitute a promising starting point for further improvements and adaptations. As awareness increases around the ecological significance of plant genetic diversity and as widespread monitoring of genetic diversity gains traction, such multispecies approaches valuably complement the tools for genetic diversity monitoring efforts.

The monitoring of plant genetic diversity (PGD) in temperate grasslands is complicated by the multiplicity of species present and by a shortage of methods for large-scale assessments. However, the continuous advancement of high-throughput DNA sequencing approaches has improved the prospects of broad, multispecies PGD monitoring. Among them, amplicon sequencing stands out as a robust and cost-effective method.

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Reference

Loera-Sánchez, M., Studer, B., & Kölliker, R. (2021). A multispecies amplicon sequencing approach for genetic diversity assessments in grassland plant species. Molecular Ecology Resources, 00, 1- 21. https://doi.org/10.1111/1755-0998.13577call_made

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Reference

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