"Ours is the first-ever structural study of this toxin," explains Alexander Belyy, first author of the study. The challenge of the project, which took a decade of work, lay in the fact that the protein is relatively large and composed of multiple modules, each devoted to a specific function. "The use of our cutting-edge cryo-EM equipment and computational power was instrumental to resolve this structure," says Stefan Raunser.
Cryo-EM allows researchers to obtain 3D images of a protein at near-atomic resolution, in this case at 3.6 Ångstrom – meaning that details 200,000 times smaller than the width of a human hair could be observed. The scientists were able to show that the structure of Mcf1 resembles a seahorse with a head containing several toxic payloads, while the tail region can attach to target cells. After the toxin is released by bacteria in the host insect, three domains in its tail region identify and bind to the target cell's membrane. Another domain of the tail then transfers the head across the membrane into the cell's cytoplasm. Once inside, the head interacts with local proteins to stimulate the release of two toxic payloads. These deadly modules disrupt the activity of essential proteins in the cell, leading to its death – and ultimately to the death of the entire insect within 24 hours of intoxication.
New organic pest control agents
Intriguingly, the researchers found that the modular structure of the tail and the initial steps of Mcf1 intoxication are highly similar to toxins from Clostridioides difficile, a human pathogen responsible for more than 120,000 hospitalizations in Europe annually. "Our study, which was originally aimed at improving biopesticides, will also have an impact on the understanding of human diseases," adds Philipp Heilen, co-first author of the study.
Looking forward, the MPI scientists want to elucidate in molecular detail how the toxic payloads of Mcf1 lead to cell death. "This new knowledge also enables engineering of highly specific insecticidal toxins," says Stefan Raunser hinting at another future direction of research: creating new toxin variants useful for ecological pest control.
