NEW ORLEANS—A novel molecular imaging agent targeting glypican-3 (GPC3) has demonstrated high sensitivity and specificity in detecting hepatocellular carcinoma (HCC), including tumors smaller than one centimeter, according to results from a pilot clinical study. The agent, 68Ga-aGPC3-scFv, coded as XH06, was shown to be safe, well-tolerated, and effective at providing high-contrast images of GPC3-positive liver tumors, offering a promising new tool for early diagnosis and staging of HCC—one of the most lethal forms of liver cancer. This research was presented at the Society of Nuclear Medicine and Molecular Imaging 2025 Annual Meeting.
HCC is the sixth most common cancer and the third leading cause of cancer-related deaths globally, accounting for 75–85 percent of all primary liver cancers. Due to its often silent progression, HCC is frequently diagnosed at an advanced stage, contributing to a five-year survival rate of just 18 percent. Most cases arise in the context of chronic hepatitis or liver cirrhosis, where underlying liver damage and fibrosis make early tumor detection particularly challenging.
"While current imaging and diagnosis of HCC primarily depend on contrast-enhanced CT or MRI to identify structural changes, PET imaging has the potential to reveal early molecular alterations that precede visible anatomical shifts," noted Mengting Li, PhD, attending physician at the Nuclear Medicine Department of Wuhan Union Hospital, in Wuhan, Hubei, China. "Our study concentrated on imaging glypican-3 (GPC3), a cell surface receptor that is overexpressed in most hepatocellular carcinomas and represents a highly specific target for molecular diagnostics and imaging."
Thirty-six patients with suspected HCC underwent 68Ga-XH06 PET/MR scans. Tumor uptake was measured, and tumor-to-liver ratios were calculated. Pharmacokinetics were assessed by analyzing tracer distribution in various organs, and safety was monitored through lab tests and vital signs. Surgical pathology was performed afterward to confirm HCC diagnoses.
68Ga-aGPC3-scFv was successfully administered to patients with no adverse effects reported. The imaging showed low background activity except for kidney accumulation, and the tracer effectively detected HCC lesions—including tumors smaller than one centimeter—with high contrast. Compared to pathology, the tracer demonstrated sensitivity of 90.63 percent and specificity of 100 percent. Tumor uptake values increased over time, confirming the probe's specificity and strong imaging performance.
"GPC3-targeted immunoPET provides clearer, more accurate imaging with high tumor-to-background contrast, enabling earlier diagnosis and better staging," said Xiaoli Lan, MD, PhD, chairwoman of Department of Nuclear Medicine at Wuhan Union Hospital. "For patients, this could mean life-saving interventions at earlier stages, improved treatment planning, and ultimately, higher survival rates. This breakthrough represents a new era in HCC diagnostics, and we are committed to accelerating its global translation, bringing earlier detection and better survival to liver cancer patients worldwide."
Abstract 252173. "GPC3-targeted immunoPET allows for early detection of HCC: a pilot clinical study," Mengting Li, Wenzhu Hu, Xiao Zhang, Rui An, Yongxue Zhang, Xiong Cai, Dawei Jiang, and Xiaoli Lan, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, China.
