Using advanced technologies, a team of scientists led by Pawel Burkhardt from the Michael Sars Centre, University of Bergen and Maike Kittelmann from Oxford Brookes University, have revealed the nervous system connectivity of ctenophores, one of the most ancient animal lineages. Reconstructing neurons from the nerve net through 3D electron microscopy, they discovered an extraordinary architecture: a continuous neural network. These findings challenge our understanding of nervous systems and their evolution.
Changing neurobiology theory
Ever since the work of scientists Santiago Ramón y Cajal and Fridtjof Nansen in the 19th century, neurobiology research is interpreted through the lens of the neuron doctrine. This theory states that nervous systems are composed of discrete individual cells.
Camillo Golgi challenged this theory by putting forward the idea that neurons within a nervous system are connected as a continuous network. Cajal and Golgi shared the Nobel Prize in 1906 for their extraordinary findings, though they were fierce competitors throughout their career.
Cajal's theory was finally proven correct by identifying neuronal junctions, so called synapses, through the invention of electron microscope in the 1950's, thereby disproving Golgi's theory. These new findings now prove that Golgi was also right.
Why ctenophores?
Ctenophores, also known as comb jellies, are fascinating organisms that have been living in the world's oceans for approximately 600 million years. When the first animals evolved, ctenophores were one of the first animal lineages on the planet. Within early evolution of neurons and nervous systems, multiple ways to make a nervous system were possibly established.
Previous attempts to describe ctenophore nervous systems connectivity had proven difficult because the organisms are delicate and very fragile, and investigating their anatomy was very challenging.
Applying new technology sparked curiosity
Pawel Burkhardt's collaboration with Maike Kittelmann, a 3D electron microscopy expert, led to the important observation that one single neuron in the ctenophore nerve net had made a small network by fusing its neuronal processes, also known as neurites, to each other.
Curious to explore this irregularity, Pawel and Maike collected a much larger 3D dataset.
"At the Centre for Bioimaging at Oxford Brookes University we have a Serial Block Face SEM (Scanning Electron Microscope) that allows automated collection of hundreds of images of one animal. One of the datasets that we now have available includes five nerve net neurons and their widely branching neurites", says Maike. Reconstructing these cells revealed an extraordinary architecture: they form a continuous neural network.
"We found fundamental differences between the nerve net of ctenophores and that of cnidarians and other animals", says Burkhardt.
"This is extremely exciting. One could argue: is it even a nervous system?" Despite its unique architecture, the ctenophore nerve net displays key characteristics found in nervous systems such as neuropeptides and ion channels generating membrane potentials.
What do the findings mean?
The characterization of the ctenophore nerve net has the potential to provide key information on the evolutionary origin of nervous systems. Through revealing the unique and unusual operating principles of ctenophore neurons, the teams offer a novel way of thinking about nervous system architectures, thereby paving the way for a new period of comparative neuroscience research.
Science Magazine 21 April 2023; https://www.science.org/
The Michael Sars Centre at the University of Bergen, Norway is an international community of scientists using advanced technologies to study the unique molecular and cellular biology of marine organisms in a changing environment. As one of the first EMBL partners, the Michael Sars Centre is rooted in the Bergen academic community and serves as a national strategical asset for Norwegian marine life sciences. The Centre aims to establish, strengthen, and leverage local, national, and international networks through specific activities, including collaborative research, joint training, and scientific exchange.
Oxford Brookes is one of the UK's leading modern universities, and is amongst the world's top universities in 19 subject areas. Set in a world-famous student city, it enjoys an international reputation for teaching excellence as well as strong links with business, industry and the public sector.
