WINSTON-SALEM, NC, April 21, 2023 – Wake Forest Institute for Regenerative Medicine (WFIRM) scientists have created a promising injectable cell therapy to treat osteoarthritis that both reduces inflammation and also regenerates articular cartilage.
Recently identified by the Food and Drug Administration as a public health crisis, osteoarthritis affects more than 520 million people worldwide who deal with pain and inflammation. Osteoarthritis is typically induced by mechanical or traumatic stress in the joint, leading to damaged cartilage that cannot be repaired naturally.
"Without better understanding of what drives the initiation and progression of osteoarthritis, effective treatment has been limited," said lead author Johanna Bolander of WFIRM. "Initially, we studied what goes wrong in osteoarthritic joints, compared these processes to functional environments, and used this information to develop an immunotherapy cell treatment."
Osteoarthritis is a disease of the joint system. The joint includes a synovial membrane – a connective tissue that lines the inner surface of the joint. The membrane functions to protect the joint and secretes a lubricating fluid filled with cell elements needed to maintain a healthy environment and to provide friction free movement.
In healthy joints when an injury occurs, the body recruits an army of inflammatory cells and sends them to the injury site to contribute to cleaning of the damaged tissues. In the osteoarthritic joint, however, a traumatic injury leads to inflammation of the synovial membrane and cartilage damage.
"With time, the inflammation worsens, leading to degradation of the cartilage lining the joint bones and chronic inflammation in the surrounding tissues. For patients, this causes severe pain, swelling and often limits daily activities," said co-author Gary Poehling, MD, an orthopedic surgeon at Atrium Health Wake Forest Baptist.
For this study, published in Science Advances, the journal of the American Association for the Advancement of Science, the research team set out to investigate what is taking place in the osteoarthritic joint environment that keeps the healing process from happening.
"We evaluated whether the cell population present in the joint fluid environment lacked the capability to contribute to functional tissue repair, or if there is something in the environment that impairs their ability to do so," said Gustavo Moviglia, PhD, a WFIRM researcher.
The team isolated cells from the joint fluid of osteoarthritic patients, separated the cells from the fluid and investigated them alone, but also in the presence of the autologous fluid. Separated from the fluid, they saw that the cells had the ability to undergo processes required for functional tissue repair. When they added a small percentage of the fluid back into the cell culture assay, the cells' abilities were impaired – they couldn't do their job – suggesting that the specific osteoarthritic environment stops them.
Based on these findings and what is known about functional tissue repair, a cell therapy was designed that can overcome the inflammatory environment and also regenerate cartilage.
"Cartilage activated immune cells that target inflammation, combined with progenitor cells aid tissue regeneration," said Anthony Atala, MD, senior author and director of WFIRM. "It's really a dynamic communication between these two cell populations that are crucial for the efficacy of the treatment."
The combination of cells leads to simultaneous treatment of several of the aspects involved in osteoarthritis: synovial inflammation, cartilage degradation, subchondral bone sclerosis and innervation of pain sensory neurons.
The therapy was tested in a pre-clinical model and was found to have the ability to reverse cartilage damage in the synovial membrane and diminish the inflammation as well. To evaluate clinical efficacy, a compassionate use study was conducted in nine patients with confirmed osteoarthritis who each received one or two injections. Efficacy was evaluated through scoring of pain and functional living, MRI scans pre- and post-treatment and a biopsy from one patient was obtained.
Once treated, the patients experienced improved quality of life, ability to participate in recreational activities, and reduced pain. Additionally, MRI studies confirmed cartilage regeneration. Additional clinical studies are required to evaluate the outcome in a larger patient population as well as to evaluate potential differences in patients in specific subgroups.
Additional co-authors include: Maria Teresita Moviglia Brandolina, Olivia Jochl, Emma Parsons, and William Vaughan, all of WFIRM.
The research was partly funded by Research Foundation - Flanders through research grant 1518618N, the postdoctoral grant 12S6817N, and the B.A.E.F. Henri Benedictus Fellowship. None of the authors have competing interests to declare.
About the Wake Forest Institute for Regenerative Medicine: The Wake Forest Institute for Regenerative Medicine is recognized as an international leader in translating scientific discovery into clinical therapies, with many world firsts, including the development and implantation of the first engineered organ in a patient. Over 400 people at the institute, the largest in the world, work on more than 40 different tissues and organs. A number of the basic principles of tissue engineering and regenerative medicine were first developed at the institute. WFIRM researchers have successfully engineered replacement tissues and organs in all four categories – flat structures, tubular tissues, hollow organs and solid organs – and 16 different applications of cell/tissue therapy technologies, such as skin, urethras, cartilage, bladders, muscle, kidney, and vaginal organs, have been successfully used in human patients. The institute, which is part of Wake Forest School of Medicine, is located in the Innovation Quarter in downtown Winston-Salem, NC, and is driven by the urgent needs of patients. The institute is making a global difference in regenerative medicine through collaborations with over 400 entities and institutions worldwide, through its government, academic and industry partnerships, its start-up entities, and through major initiatives in breakthrough technologies, such as tissue engineering, cell therapies, diagnostics, drug discovery, biomanufacturing, nanotechnology, gene editing and 3D printing.
