Oak Brook, IL – The July 2023 issue of SLAS Discovery, the open access journal focused on research progressing drug discovery, is now available. Volume 28, Issue 5, contains one short communication and four full-length articles covering assay quality metrics, fluorescence resonance energy transfer (FRET) and small molecule glycomimetics and other high-throughput screening-related research.
Full-length articles
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Mathematical relationships between control group variability and assay quality metrics
Authored by early career researcher Andrew Lim, Ph.D. (ALBORADA Drug Discovery Institute), this article assesses the relationships between control group variability and assay quality metrics to understand the factors that improve assay quality metrics by identifying which variables are having the most impact.
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Fluorescent probe for the identification of potent inhibitors of the macrophage infectivity potentiator (Mip) protein of Burkholderia pseudomallei
In this study, a fluorescence polarization (FP) assay was established to enable the screening and effective development of Burkholderia pseudomallei macrophage infectivity potentiator (BpMip) inhibitors. The presented assay may potentially contribute to the development of novel therapeutics against melioidosis (Whitmore's disease) and other microbial diseases caused by Mip-bearing pathogens.
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HTS driven by fluorescence lifetime detection of FRET identifies activators and inhibitors of cardiac myosin
This team of researchers developed technology for high-throughput time-resolved fluorescence lifetime detection of fluorescence resonance energy transfer (FRET), which enables the detection of allosteric modulators by monitoring changes in protein structure.
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Evaluating the affinity and kinetics of small molecule glycomimetics for human and mouse galectin-3 using surface plasmon resonance
The researchers explored the use of surface plasmon resonance (SPR) for compound screening by comparing human and mouse galectin-3 affinity measures between FP and SPR assays.
Short Communications
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Label-free high-throughput screening via acoustic ejection mass spectrometry put into practice
The results of this study confirm that acoustic droplet ejection-open port interface-mass spectrometry (ADE-OPI-MS) is a suitable and effective method for identifying chemical starting points in high-throughput screening (HTS) lead generation projects.
Access to the July issue of SLAS Discovery is available at https://www.slas-discovery.org/issue/S2472-5552(23)X0006-9
