Jülich / Garching, 23 February 2022 - The brain is the centre of our nervous system - structural changes are often involved in neurological diseases and mental disorders. A team from Forschungszentrum Jülich has now developed a neutron-based method at the Heinz Maier-Leibnitz Zentrum (MLZ) to study brain slices and gain a better understanding of these types of diseases.
The brain can be divided into so-called grey matter and white matter. The white matter contains the axons that transmit stimuli. For faster stimulus transmission, the axons are wrapped in an insulating layer of myelin, in the same way as a cable is wrapped in rubber insulation to ensure that no electricity is lost along the way.
3D polarized light imaging scan of a mouse brain. The structural information about the orientation of the nerve fibres from this image served as a comparison for the new neutron measurements.
Copyright: Axer, Amunts et al. / INM-1, Forschungszentrum Jülich
If the myelin sheath is damaged or degraded, this leads to impaired brain and body functions. In multiple sclerosis, for example, the insulating myelin layer is severely impaired. However, the exact causes of the disease are still unclear.
Researchers at the Jülich Institute of Neuroscience and Medicine and the Jülich Centre for Neutron Science have developed a new imaging method at the small-angle scattering facility KWS-1 at the MLZ to map the density, structure, and spatial orientation of nerve fibres and myelin.
New method complements previous procedures
"For a long time now, it has been possible to determine the spatial orientation of nerve fibres in brain slices using light microscopy and polarization," explains Dr. Heinrich Frielinghaus from the JCNS, head of the KWS-1 group, "but the exact structure and orientation of the myelin on the molecular scale cannot be detected using light." With the help of neutrons, the researchers can now for the first time determine both structure and orientation, and correlate these with polarization measurements.
Schematic diagram of the experimental setup at the small-angle scattering instrument. The neutron beam hits a brain section ("sample"). A detector records the scattering.
Copyright: Forschungszentrum Jülich
The special feature of this new method is that it allows scientists to image the axons bundled into nerve fibers at the same time as the insulating myelin sheath.
"With our neutron-based method, we can confirm the results of previous methods and provide additional information," says Frielinghaus.
Applications in medicine
"Since we work with brain slices, it is of course not a diagnostic procedure," explains Frielinghaus. With their new method, the researchers hope to better understand the causes of neurological diseases in the future by being able to visualize structural changes in the brain more completely.
Dr. Heinrich Frielinghaus at the small-angle scattering facility.
Copyright: Andreas Heddergott/TUM
The resolution of the new method is still in the millimeter range. However, Frielinghaus and his colleagues expect that measurements precise to the micrometer will be possible in the future. This will enable them to study even the smallest structures in biological tissues.
Original publication
S. Maiti, H. Frielinghaus, D. Gräßel, M. Dulle, M. Axer, S. Förster, Distribution and orientation
of nerve fibers and myelin assembly in a brain section retrieved by small‐angle neutron scattering, Sci Rep 11 (2021) DOI: 10.1038/s41598-021-92995-2
Further Information
Jülich Centre for Neutron Science
Institute for Neuroscience and Medicine - INM-1, Fibre architecture
