Using innovative RNA technology, Würzburg researchers have intervened in the reproduction of phages. The study, published in Nature, could pave the way for new therapeutic approaches to treating infections.
Summary
Phages are viruses that infect bacteria. Using phages therapeutically could be very useful in fighting antibiotic-resistant pathogens, but the molecular interactions between phages and host bacteria are not yet sufficiently understood. Jörg Vogel's research group at the Helmholtz Institute for RNA-based Infection Research (HIRI) and the Institute of Molecular Infection Biology (IMIB) in Würzburg has now succeeded in specifically interfering with phage reproduction using a molecular tool called antisense oligomers (ASOs). This innovative RNA technology offers new insights into the molecular world of phages and is expected to advance the development of future therapeutic applications. The study has been published in the journal Nature.
Phages Protect Human Health
Like humans, bacteria have to cope with viruses-known as bacteriophages, or phages for short. Phages invade bacteria, hijack their cellular machinery, multiply, and cause the bacterial cell to burst. This releases new phages, which then go on to infect other bacteria.
Phages are harmless to humans because they target only bacteria. They are also quite selective. Most phages are specialized to infect specific host bacteria, including bacterial pathogens.
"By attacking and decimating pathogens, phages protect our health as a side effect-in a kind of covert operation, so to speak. Harnessing their therapeutic potential, especially against the backdrop of increasing antibiotic resistance, would be a game changer," says Jörg Vogel, lead author of the study. Vogel is the founding director of the Helmholtz Institute for RNA-based Infection Research (HIRI) in Würzburg, a site of the Braunschweig Helmholtz Centre for Infection Research (HZI) in cooperation with Julius-Maximilians-Universität Würzburg (JMU). He also heads the Institute of Molecular Infection Biology (IMIB) at the JMU Faculty of Medicine.
Molecular Tool Introduced - Phage Manipulated
"In order to use phages therapeutically, we need a much better understanding of the molecular interaction between phages and host bacteria," says Milan Gerovac, the study's first author. Gerovac was a postdoc in Vogel's laboratory and now runs an independent research group "Complexes in Phage-Infected Cells" at the HZI. "Not much is known about this. One reason being that phages protect their genetic material from cellular defense systems of the bacteria with a kind of protective shield. Unfortunately, this shield is also quite effective against common molecular investigation methods."
To decipher the molecular phage-host relationship, they need a new approach-and that is exactly what the HIRI researchers did in their current study. With an innovative RNA-based molecular tool known as antisense oligomers (ASOs), they succeeded in interfering specifically with the phage reproduction cycle.
"The ASOs introduced into the bacterial cell switched off the synthesis of specific phage proteins at a key point," Gerovac explains. "We were able to 'hack' into phage replication with the ASOs, so to speak."
Antibacterial ASOs, also known as programmable antibiotics or asobiotics, have been studied for some time in Vogel's laboratory. "Since ASOs are known to inhibit protein synthesis in bacteria, we suspected that they could also do so in phages. This is because phages reproduce with the help of the host bacteria's cellular machinery," says Vogel. "And we were absolutely right!"
In Focus: A Jumbo Phage That Kills Hospital Germs
Using ASO technology, the researchers successfully prevented phage propagation in various phage-bacteria pairs, demonstrating that the approach is broadly applicable.
Their research focused on a jumbo phage called ΦKZ that could potentially treat dangerous infections of wounds, airways, and lungs caused by the hospital germ Pseudomonas aeruginosa.
"Jumbo phages have a very large genome," Gerovac explains. "With the help of ASOs, we were able to systematically switch off the synthesis of a large number of phage proteins. Using this knock-down screening approach, we identified previously unknown proteins central to phage propagation."
The researchers hope that ASO technology will be widely used in phage research to better understand the fundamental molecular mechanisms of phages and advance the development of new therapeutic approaches in the fight against bacterial pathogens.
Funding
The study was funded by the German Research Foundation (DFG) in the priority program "New Concepts of Virus-Host Interaction in Prokaryotes - From Single Cells to Microbial Communities" and by a Gottfried Wilhelm Leibniz Prize (both awarded to Jörg Vogel). Vogel is also a member of the Würzburg-Munich DFG Cluster of Excellence NUCLEATE and the BMBF Cluster CNAT-M.
Publication
Gerovac M, Buhlmann L, Zhu Y, Ðurica-Mitić S, Rech V, Carien S, Gräfenhan T, Popella L, Vogel J: Programmable antisense oligomers for phage functional genomics, Nature (2025), DOI: 10.1038/s41586-025-09499-6
Helmholtz Institute for RNA-based Infection Research
