The H5N1 strain of highly pathogenic avian influenza H5N1 has been detected in over 700 herds of dairy cows in California, the largest dairy-producing state in the U.S. A study published May 5th in the open-access journal PLOS Biology led by Seema S. Lakdawala at Emory University School of Medicine, U.S. and Jason Lombard at Colorado State University, U.S. suggests that avian influenza (H5N1) is transmitted through multiple, previously unknown sources and that some H5N1 positive cows do not show clinical signs of infection.
H5N1 may spread on dairy farms through direct contact with unpasteurized milk, such as via contaminated milking equipment. However, the full picture of how the virus can be spread on farms with infected cows is unclear. In order to better understand transmission routes of avian flu on dairy farms, researchers took air, farm wastewater, and milk samples on fourteen dairy farms testing positive for H5N1 across two different California regions between October 2024 and January 2025. They tested all samples for the presence of virus and performed genome sequencing on detected virus to identify any genetic variants and mutations.
The researchers detected airborne virus from the exhaled breath of infected cows and in the dairy parlor, identified the presence of virus in the wastewater, and found a high prevalence of cows who tested positive for H5N1 despite being asymptomatic.
The extensive environmental contamination of infected dairy farms suggests a higher risk of viral spread from cows to humans and other animals. However, future studies are needed to validate these results as longitudinal sampling of individual cows was limited to only fourteen animals. Sampling across a larger sample of farms over a longer time period is also needed to support the findings.
Dr. Lakdawala notes, "Our data confirm the presence of infectious H5N1 virus in the air and reclaimed farm wastewater sites. In addition, we observed high viral loads and H5 antibodies in the milk of cows, including those without clinical signs, suggesting that multiple modes of H5N1 transmission likely exist on farms. These results identify additional sources of viral exposure for cows, peridomestic wildlife, and humans, highlighting the need for multiple mitigation strategies to reduce the spread of H5N1 within a herd and to humans."
The authors add, "Detection of infectious virus in the air and waste streams on farms was surprising but highlights that there is a considerable amount of infectious virus on farms and multiple sources of infection exist."
"Targeted interventions in the dairy parlors to reduce the amount of aerosols in the air and inactivation of sick milk prior to disposal will provide additional barriers to infection of farm workers and likely other farm animals."
In your coverage, please use this URL to provide access to the freely available paper in PLOS Biology: https://plos.io/4sspx4Z
Citation: Campbell AJ, Shephard M, Paulos AP, Pauly MD, Vu MN, Stenkamp-Strahm C, et al. (2026) Surveillance on California dairy farms reveals multiple possible sources of H5N1 influenza virus transmission. PLoS Biol 24(5): e3003761. https://doi.org/10.1371/journal.pbio.3003761
Author countries: United States of America
Funding: This work was supported by discretionary funds to S.S.L from Emory University and gift funds to the Emory Center for Transmission of Airborne Pathogens, provided by the California Dairy Research Foundation ( https://cdrf.org/ ) and Flu Lab, a California-based organization founded to advance innovative approaches for the prevention and treatment of influenza ( https://theflulab.org/ ). A.S.L., W.J.F., and E.E.B. were supported by the Michigan Infectious Diseases Genomics Center (NIAID U19 AI181767). This work was also supported in part by a gift to M.K.W. from the Sergey Brin Family Foundation. Part of this publication was made possible, in part, by an Agreement from the United States Department of Agriculture's APHIS to J.L. This publication may not necessarily express the views of APHIS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
